ZEISS Microscopy welcomes you to MMC 2019 in Manchester this July. Visit us on stand 610 and be one of the first to experience our new ZEISS LSM 980 with Airyscan 2.
MMC 2019 is set to be as big and bold as ever before with 36 conference sessions, an exhibition with more than 90 companies represented, a brilliant selection of features such as pre-event workshops and turn-up-and-learn training opportunities and a busy social programme.
Pre bookable on-stand demonstrations are available for both the GeminiSEM 450 and the NEW LSM 980 with Airyscan 2, with various time slots available through the congress. These sessions are designed for you and up to 2 colleagues and aim to help you get hands-on with the latest technology, using your own samples if you wish. You will receive expert advice from our applications specialists who will be on-hand to take you through the system and discuss any specific requirements that you may have.
Please use the links below to reserve your preferred time slot - allocated on a first-come-first-served basis!
Not sure about your schedule for MMC yet? No problem - a small number of demonstration slots will also be available on the day, offered on a first-come-first-served basis. Simply visit us on the stand to check availability.
Discover the new LSM 9 family for confocal 4D imaging with high sensitivity and spectral flexibility. Experience the new Multiplex mode for parallel pixel acquisition on Airyscan 2, for gentle and fast confocal imaging - with acquisition times shorter than ever before.
The GeminiSEM family stands for effortless imaging with sub-nanometer resolution and high detection efficiency, even in variable pressure mode. Put the GeminiSEM 450 to the test - your specialist for speed and surface sensitivity.
Run by ZEISS applications specialists, our programme of exciting talks will give you the opportunity to learn more about advances in correlative microscopy techniques, live cell imaging, machine-learning-based segmentation, confocal cryo fluorescence imaging and collaborative image processing.
There's no need to pre-register for any of the sessions, just turn up at the start time indicated below. Full details including the date and time, location and abstract can be found below.
|Enabling Deeper Insights in Structural Biology Using Cryo-Correlative Workflows|
|Tuesday 2nd July||12:00 - 12:30||Workshop Room 2|
The investigation of vitrified biological specimens enables the visualization of cellular ultrastructure in a near-to-native fully hydrated state, unadulterated by harmful preparation methods. With light and electron microscopy in combination functional and structural information from the sample can be extracted. Here, we focus on two recent cryo imaging modalities and discuss their impact on cryo correlative workflows. First, we present confocal cryo fluorescence microscopy, utilizing the Airyscan detector with improved signal to noise ratio (SNR) and resolution. Second, we show volume imaging of different biological specimens by focused ion beam scanning electron microscopy (FIB/SEM) under cryo conditions.
Confocal laser scanning microscopes (LSM) are renowned for their optical sectioning capability, a feature enabled by utilizing a pinhole that rejects out of focus light. Closing the pinhole improves lateral resolution, but also causes less light to reach the detector leading to reduced signal to noise ratios (SNR). In cryo fluorescence microscopy, the situation is aggravated by the fact that currently no immersion optics are readily available and consequently only numerical apertures below NA 1 are possible. We combined the Airyscan detector together with a cryo correlative stage for fluorescent imaging of vitrified samples. The Airyscan detection module allows the spatially resolved detection of fluorescence light otherwise rejected by the pinhole in a standard confocal system. We demonstrate that even without immersion optics, Airyscan achieves a significant increase in resolution and SNR compared to standard confocal images.
FIB/SEM tomography enables the acquisition of large three-dimensional volumetric data from biological specimens by sequentially removing material with the ion beam and imaging the exposed block faces with the electron beam. This imaging method can be applied to frozen hydrated specimens, as recently demonstrated in (J Struct Biol. 2013 Nov; 184(2):355 60. doi: 10.1016/j.jsb.2013.09.024). In contrast to Cryo TEM Tomography (cryo ET), FIB/SEM tomography allows easy mapping of large multicellular specimens in the near native state and is particularly suited to analyse samples that require vitrification. The trade-off compared to cryo ET is a somewhat lower resolution comparable with resin-embedded samples. FIB/SEM volume imaging however elegantly extends cellular cryo ET by providing much larger volume access at significantly reduced preparative labour and can thus add necessary contextual information that broadens the view possible with cryo ET.
Both methods by themselves promise significant advantages for biomedical research by enabling the investigation of biological specimens in the near native fully hydrated state. Yet correlating both imaging modalities, LSM and FIB/SEM of vitrified samples, has the potential to provide even deeper insights into biological context. Our new software module, ZEN Connect, allows the user to perform one concise workflow between a LSM and FIB/SEM instrument by keeping the various images organized spatially and at scale in one comprehensive project.
The correlation between cryo light and electron microscopy data will greatly benefit from the ever-increasing resolution in fluorescence imaging. Cryo Airyscan is the next step into that direction delivering three-dimensional optical sectioning data that enables reliable targeting of cellular structures in a FIB/SEM microscope. Once identified, the structural context of the target location either is explored by volume imaging or can be processed further as a cryo lamella for subsequent cryo TEM Tomography.
Alexandra Elli, Schertel Andreas & Niyaz Yilmaz - Carl Zeiss Microscopy GmbH
|New Methods for Fast and Gentle Live Cell Imaging in 3D|
|Tuesday 2nd July||14:00 - 14:30
||Workshop Room 1|
Researchers often need to image the smallest structures, catch the faintest signal or track the fastest processes – or do all of that at once. When it comes to getting accurate data from live cells or other weakly-labelled samples, there is no such thing as too much sensitivity, resolution or speed. Each photon of emission light is precious. At ZEISS Microscopy, we constantly strive to develop light efficient imaging technologies. ZEISS Airyscan and Lattice SIM allow researchers in facilities and single labs to get as much information about their challenging samples as possible. Our presentation will give an overview about new developments in the field of gentle and fast optical sectioning techniques with superresolution. A number of challenging application examples will be presented and discussed.
Joseph Huff, PhD - Application Development for Life Sciences - Carl Zeiss Microscopy GmbH
|Multimodal Microscopy for Very Large 2D & 3D Imaging|
|Wednesday 3rd July
||14:00 - 14:30||Workshop Room 1|
Modern microscopy labs are typically outfitted with a suite of instruments, capable of capturing data across a range of length scales in 2D- and 3D, from the centimeters to the sub-nanometers. These imaging instruments are often complimented by analytical techniques, such as spectroscopic chemical characterization platforms or mass spectrometry and are designed to produce a comprehensive depiction of the material under investigation. Recently, a novel multi-beam SEM (MSEM) technology for imaging of large sample areas has been developed by ZEISS. The MultiSEM family features 61 or even 91 electron beams scanning in parallel, resulting in an imaging throughput of up to 2 TeraPixels per hour [s. ref.] is now achievable, therefore enabling extremely large-scale imaging experiments in 2D and 3D.
Here, we present a unique advancement enabling correlative microscopy, which uses a centralized software platform to pull together data from light-, electron-, Ion-, and X-Ray Microscopy (XRM). Beyond just correlating the various datasets, the approach allows data from one technique to be used to drive the hardware in another technique, facilitating easy transfer of information between the suite of available microscopes and the operator. The presentation will give an overview of the current state of the technology, its potential application space and the challenges in data handling imposed by the enormously increased data rate.
Antonio Casares - Carl Zeiss Microscopy GmbH
|From Nanoparticles to Neuron Segmentation: Accelerating Research Through Digital Technology|
|Wednesday 3rd July
||14:30 - 15:00
||Workshop Room 1|
Manual tasks are slowing research down! Even in today's digital world microscopists still rely on manual steps for repetitive, routine tasks, such as image segmentation, analysis, and reporting. Also, an average microscopist uses at least 3 different software applications that don't talk to each other. Manual tasks combined with fragmented software makes experiments not only slow but also unrepeatable.
In this talk, we present an online platform (APEER) to address the above-mentioned concerns. APEER leverages state of the art digital technologies to accelerate research via workflow customization and automation. The effectiveness of APEER will be illustrated using two challenging examples, nanoparticle and neuron segmentation, respectively.
|Solutions for the Challenges of Advanced FIB-SEM Tomography Including Machine Learning Based Segmentation|
|Thursday 4th July
||11:30 - 12:00||Workshop Room 2|
Focused ion beam scanning electron microscopy (FIB-SEM) tomography enables high-resolution, site-specific characterization in 3D. It can even be combined with analytic mapping methods like energy dispersive x-ray spectroscopy (EDS) or electron backscatter diffraction (EBSD). In an iterative process, the ion beam removes a slice of material before SEM image and EBSD map are acquired. This way a data stack is generated slice by slice that can be used afterwards to reconstruct the information about the volume of interest. However, this workflow holds several challenges that need to be solved to acquire a high-quality, consistent dataset. These challenges will be discussed, and solutions will be presented.
After the data acquisition, 3D reconstruction and image segmentation are the next important steps. Dedicated solutions to facilitate these will be presented as well. Especially when it comes to image segmentation, machine learning based methods have shown great potential but are usually not straight-forward to apply. Therefore, a new software module will be introduced, which was designed with ease-of-use in mind and enables machine learning based segmentation for everybody.
Image segmentation and analysis are currently two of the biggest challenges in microscopy - segmentation lays the foundation for all subsequent image analysis steps. Scroll down to learn more about our digital station on the MMC stand, featuring ZEISS ZEN Intellesis and APEER, which are both designed to help you address the challenges of image segmentation and analysis.
Modern ZEISS light, electron and X-ray microscopes produce large volumes of imaging data that span from the gigabyte up to the terabyte range. Thanks to innovative software solutions by ARIVIS, it is now possible to visualise them either as volumes or segmented surfaces with virtual reality headsets.
For MMC we've partnered with The Natural History Museum to bring you some fascinating sample datasets acquired on ZEISS systems, which we invite you to come and try out the immersive experience for yourself! Navigate the samples by moving your head and hidden parts of the sample can be seen, discussed and analysed.
ZEISS ZEN Intellesis uses deep learning and Python to easily create robust and reproducible segmentation results, even for non-experts. You can now train the software once and then ZEN Intellesis can segment a batch of hundreds of images automatically.
On the ZEISS stand at MMC you will be able to experience applications for both life sciences and materials sciences. Can't wait until then? Click the link below to find out more and to take advantage of our free 30-day trial, with access to the full software functionality.
Researchers can be working with up to five different software solutions creating workflows to generate the quantitative data that projects need - but we want to change that! APEER is an initiative started by ZEISS to create collaborative and customizable solutions in the microscopy community. This new digital platform enables you to automatically process images in the cloud by leveraging the application workflows for 3D reconstructions, staining or segmenting.
Join us on the ZEISS stand as we demonstrate different workflow examples, from image acquisition to particle analysis and reporting.
On the ZEISS stand at MMC 2019, we will be showcasing a wide range of imaging and analytical systems; from SEM and light microscopy through to confocal and automated systems. Scroll down to learn more about some of the machines.
The GeminiSEM family stands for effortless imaging with sub-nanometer resolution and high detection efficiency, even in variable pressure mode. Try out the GeminiSEM 450 - your specialist for speed and surface sensitivity.
Pre-book your personal demonstration at MMC via the link above or visit our stand and experience the system at MMC.
The new LSM 9 family with Airyscan 2 represents the latest step in confocal microscopy. Acquisition strategies of the new Multiplex mode enable you to leverage the unique combination of optically sectioned superresolution and sensitivity.
An LSM 980 system will be available on the ZEISS stand at MMC to try, with pre-bookable appointments offered via the link above.
Building on industry-best resolution and contrast, the ZEISS Xradia Versa Family expands the boundaries of your non-destructive sub-micron scale imaging, without sacrificing resolution and contrast.
Speak to one of our experts at MMC to find out how you can unlock new degrees of versatility for your scientific discovery and industrial research.
Exhibition Dates & Opening Hours:
Tuesday 2nd July: 09:15 - 18:00
Wednesday 3rd July: 09:15 - 18:00
Thursday 4th July: 09:00 - 15:00
Manchester Central Convention Complex
Windmill Street, Manchester
Free tea and coffee is available all day from the central catering point in the exhibition hall. There will also be a number of free water points around the exhibition. Breakfast, lunches and snacks will be available on a cash basis from the catering areas within the exhibition from Tuesday to Thursday.