Half-Day Online Symposium: Future Perspectives in Live Imaging

Aspect

Title

Session Lead

Timing

Event Start

Welcome

Rosy Manser (ZEISS Microscopy)

Start - 00:01:10

Keynote Talk and Q&A

Into the Future: Quantitative Live Cell Imaging of the Immune Response​

New perspectives of the immune response are currently emerging. Increasing evidence indicates that immune cells dynamically adapt their signalling and mechanics to external stimuli. Quantifying these processes have therefore become a contentious challenge in the biomedical sciences, which has only recently become possible through bioimaging. However, many opportunities for exploration using imaging are limited by the impact of the invasiveness and phototoxic burden to the living cells. This talk discusses our route to success and also will take a look towards future possibilities for minimal invasive imaging to transform our understanding of the immune cells in a quantitative way.

Marco Fritzsche (University of Oxford)

Keynote: 00:01:10 - 00:30:05

Q&A: 00:30:05 - 00:44:05

Breakout Session 1: Management Of Samples And Live Imaging Technologies

Stream A: Managing Cutting Edge Live Imaging Technology​
• Build or buy – the pros and cons​
• Practical considerations for infrastructure for data analysis and management​
• Optimising/tracking experimental variations

Kevin O’Holleran (University of Cambridge)

00:44:05 - 01:15:05

Stream B: ​Sample Preparation and Experimental Set Ups for Pushing the Limits of Live Imaging ​
• Sample mounting considerations and optimisation​
• Minimising phototoxicity in live samples​
• Variables for selecting the best technology for each experiment

Nick van Hateran (University of Sheffield)

01:15:05 - 01:46:07

Stream C: Complexities Associated With Working With Infectious Organisms ​
• How dangerous is your bug and what are the containment considerations? ​
• Does your institution enable live infections on the microscope?​
• Safety considerations for users, particularly using mixed organisms

Theresa Ward (London School of Hygiene & Tropical Medicine)

01:46:07 - 02:17:22

Consolidation

Summary of Key Discussion Points From the First Breakout Sessions

02:17:22 - 02:26:26

Keynote Talk and Q&A

Pushing the Limits of Live Cell Imaging to Study Surface Protein Trafficking in Yeast​

Capturing the dynamics of membrane trafficking in yeast poses many challenges, particularly when planning experiments with endogenous levels of protein expression. Developments in technology have enabled a steady increase in acquisition speed, resolution and duration in recent years and this has resulted in a better understanding of some of the key trafficking processes. Optimising experiments is no easy task and this talk will provide an overview of the challenges associated with sample management and provide some insights into how to opitimise experiments with different technologies when imaging yeast. ​

Chris MacDonald (University of York)

Keynote: 02:26:26 - 02:59: 30

Q&A: 02:59: 30 - 03:08:06

Breakout Session 2: Configuration and Experimental Design of Complex Live Experiments

Stream D: ​Multimodal Imaging to Drive Live Acquisition Possibilities ​
• Technical opportunities and considerations​
• What kind of imaging approaches can be combined and to what end?​
• How can you combine the datasets to ensure correct interpretation?

Michelle Peckham​ (University of Leeds)

03:08:06 - 03:41:43

Stream E: ​Imaging Biology From the Single Cell to the Whole Animal ​
• Live imaging across scales: Bacteria, cells, whole animals​
• High content, high resolution: What is future microscopy?​
• Choosing the right animal model for your research question

Serge Mostowy ​(London School of Hygiene & Tropical Medicine)

03:41:43 - 04:10:40

Stream F: ​Considerations When Addressing Questions Using in Vivo Imaging​
• Which animal model & fluorophores should you choose?​
• Selecting the right instrument for your experiment​
• Approaches to ensure data confidence and controls

Lead: Yi Feng ​(University of Edinburgh)

Panellists: Leo Carlin (The Beatson Institute for Cancer Research), Rebecca Richardson (University of Bristol)

04:10:40 - 04:43:17

Consolidation

Summary of Key Discussion Points From the Second Breakout Sessions

04:43:17 - End