Lamin B1 is involved in disassembling and reforming the nuclear envelope during mitosis. The formation of so-called ‘nuclear invaginations’ has been reported frequently during mitotic events. However, most research so far on these unique structures has been done with fixed cells and as a result their function is largely unknown.

The data observes cells going through mitosis throughout the whole duration. The formation and dynamics of nuclear invaginations can clearly be observed in most of the cells, throughout the complete cell cycle. The gentleness of Lattice Lightsheet 7 imaging enables imaging of mitotic events over longer time periods, preventing arrest from excitation light. Combined with the fast volumetric imaging and near-isotropic resolution the ZEISS Lattice Lightsheet 7 is the perfect tool for challenging experiments like this.