ZEISS Elyra 7 with Lattice SIM²

London School of Hygiene & Tropical Medicine ELYRA 7 Seminar

The super-resolution microscope Elyra 7 takes you far beyond the diffraction limit of conventional microscopy: With Lattice SIM² you can now double the conventional SIM resolution and discriminate the finest sub-organelle structures, even those no more than 60 nm apart. You don‘t need to sacrifice resolution when imaging at high speed using only the minimal exposure needed for life observation. Elyra 7 enables you to combine super-resolution and high-dynamic imaging – without the need for special sample preparation or expert knowledge of complex microscopy techniques.

Webinar: Zeiss Elyra 7 - Exploring New Frontiers in Live Cell Imaging

In this talk we will discuss the latest innovations in the ZEISS Elyra 7. Lattice SIM2, unlocks new imaging capabilities, extending the capabilities of super resolution microscopy down to 60 nm xy resolution in fixed and live samples. We will also explore the speed and flexibility of the Elyra 7, with Lattice SIM2 and SIM2 Apotome offering powerful imaging capabilities across different magnifications.

Finally, we'll discuss how to best prepare your samples for the ZEISS Elyra 7 to get the most out of system.

Date: Thursday 24th February 2022
Time: 10:30 – 11:30
Speaker: Jon Shewring, Product and Application Sales Support Specialist, ZEISS.

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Typical Applications

Live Cell Imaging

SIM2

  • Reveal mechanistic details in live cells, e.g. moving organelles, vesicle trafficking, membrane reorganisation
  • Resolve structural details in 3D and multiple colours. Acquire up to four colours with optimized resolution for each wavelength.
  • Discover fast cellular processes in the context of whole cells.
  • Observe fast dynamics of fine structures without perturbing the specimen

SMLM
 
  • Track many molecules over a large FOV and retrieve diffusion behaviour information in entire cells. 
  • Study molecular level structural changes of sub-minute-scale dynamic processes, e.g. mechanisms of focal adhesions, reorganization of tubulin, vesicle shuttling.

Small Evolving Organisms

  • Resolve structural detail in 3D with high penetration depth
  • Resolve structural details in 3D over large areas using tiling

Fixed Specimens

SIM2
  • Probe the structural organization of a whole cell with the advantage of fluorescence specificity and superresolution.
  • Investigate arrangement of cellular components and proteins
  • Explore interaction of molecules.
SMLM
  • Reveal the ultrastructue of organelles and molecular assemblies
  • Explore interaction of molecules

ZEISS Elyra 7 at Work

 

U2OS cell expressing Rab5-mEmerald (green) and tdTomato tagged Golgi associated transport marker (magenta). Simultaneous dual-color acquisition with an exposure time of 1.5ms / phase for a FOV of 1024 × 1024 pixel (64 µm × 64 µm).

Burst Mode - Super-resolution Imaging at up to 255 fps

The diffusive and especially the ballistic movement of small vesicles in cells can be captured only when super-resolution and high-dynamic imaging are possible at the same time. With the Burst processing of 2D time lapse data, Elyra 7 is able to generate super-resolution images at 255 Hz in a large field of view and even acquire two colors simultaneously in both Lattice SIM and SIM Apotome acquisition modes.

Architecture of threefold labeled synaptonemal complexes from mouse testis visualized via immunolabeling of SYCP3 with SeTau647, SYCP1-C with Alexa 488 and SYCP1-N with Alexa 568 and Lattice SIM² mode

Multi-color Super-resolution Imaging for Conventionally Stained Samples

Due to its small size, three-color imaging of the synaptonemal complex has previously been possible only using complex methods like super-resolution imaging of three-fold expanded samples. Lattice SIM² resolves the two strands of SYCP3 (lateral elements) as well as SYCP1-C (C-terminus of transverse filaments) without special sample treatment or staining for distances well below 100 nm. More importantly, the three-color image provides structural information about the distances between the proteins SYCP3 and SYCP1. Even within the SYCP1 protein, the different labeled N- and C-Terminus can be clearly separated with less than 50 nm resolution between the two labels.

 

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