ZEISS Celldiscoverer 7 with LSM 900 and Airyscan 2

Your automated microscope for gentle and fast confocal 4D imaging

With Celldiscoverer 7, you combine the ease-of-use of an automated high-end imaging system with the image quality and flexibility of a classic inverted research microscope. Celldiscoverer 7 calibrates itself, detects and focuses on your samples while the optics adjust themselves.

Life sciences research often calls for optical sectioning to image samples with best possible contrast and resolution. The Celldiscoverer 7 with LSM 900 and Airyscan 2 enables gentle 3D imaging of dynamic processes in your living samples with highest framerates in superresolution and easy separation of multiple labels with spectral imaging.

Reproducible Results Made Easy

Find Focus – Keep Focus

A hardware-based focus finds and keeps the focus automatically after detecting the thickness and optical properties of the sample carrier for demanding long-term, time-lapse imaging.

Multimodal imaging

A system with a fast, sensitive sCMOS camera combined with the LSM 900 and Airyscan 2. Ensuring a suitable modality to suit an array of biological questions.

Easily Get Reproducible Results

Automatic calibration routines ensure reproducible results in live cell imaging.

Autoimmersion

Celldiscoverer 7 combines an outstanding 50x water immersion objective with rapid automated immersion supply and removal. Automatic monitoring of immersion and adding water, as needed

Automatic Sample Recognition

You’re free to use Petri dishes, chamber slides, multiwell plates, plastic or glass, thin or thick vessel bottoms, low skirt or high skirt plates. Automatic sample recognition detects vessel features and calibrates the microscope for optimal performance.

Autocorr Objectives

Autocorr objectives automatically adjust the correct collar to correct spherical aberrations delivering you crisp contrast and high resolution

Case Study: Life with the Celldiscoverer 7

Discover how the Celldiscoverer 7 is changing research

We spoke with Serge Mostowy, Professor of Cellular Microbiology, and his team at the London School of Hygiene and Tropical Medicine, UK to uncover the transformative impact of the ZEISS Celldiscoverer 7 on their research.


Whitepaper:

The transformative impact of the ZEISS Celldiscoverer 7

The ZEISS Celldiscoverer 7 was first installed in the Mostowy Lab at the London School of Hygiene & Tropical Medicine in 2019. Since then, the system has enabled them to evolve both their research and imaging related questions - download the White Paper to find out more.

From Image to Results

Image analysis? No Problems

ZEISS Celldiscoverer 7 is an automated live cell imaging system designed to make complex microscopy simple enabling high-throughput applications, but what about the data? With easy to develop Image Analysis Pipelines in arivis Vision4D you can even tackle those samples which traditionally produce high demand on analytical techniques. Once a pipeline has been created and optimized, it is then possible to scale up the analysis by use of the server-based VisionHub platform enabling users to run batch analysis and have results readily available within the web-based user interface.

In our new series “From Image to Results”, explore how data captured with the ZEISS Celldiscoverer 7 can be combined with a powerful Image Analysis Pipeline delivered by arivis Vision4D to scale up analysis of organoid formation.

Typical Applications

Live Cell Imaging

  • Short-term or long-term observation of physiological and morphological parameters in 2D/ 3D during growth, differentiation, motility and interaction.
  • Acquire high resolution images of multi-labelled cell culture from multiwell plates quickly.
  • Study the motility of vesicles, organelles and subcellular structures
  • Examine the interplay of multiple proteins
  • Image subcellular structures at physiological expression levels

  • Study molecular dynamic with FRAP
  • Explore the interaction of two proteins with FRET
  • Observation of stimulus-induced responses of cells or organisms without disturbing the environmental control.
  • Perform label free growth curve assays over several days

3D Cell Imaging

  • Live imaging of spheroids or organoids
  • Organoid self-organization
  • Cell migration and proliferation within organoids
  • Study morphogenetic process using tubule organoids.

Small Evolving Organisms

  • Cell migration and proliferation within organoids
  • Study morphogenetic process using tubule organoids.
  • Analyse the embryogenesis of small model organisms

Fixed Specimens

  • Change quickly between large overview scans and high resolution imaging
  • Fixed fluorescently labelled tissue, cell culture samples - Identification, quantification and qualification of cell types, pathological and pharmacological pathways using cell-, tissue and protein markers in 2D and 3D samples.
  • Label-free fixed and thin tissue slices or small organisms

ZEISS Celldiscoverer 7 at Work

Kidney tubule organoids grown from mIMCD3 cells © Michael Koettgen & Lukas Westermann; ZKF Freiburg, Germany
Kidney Tubule Organoids grown from mIMCD3 cells, Plan-APOCHROMAT 20x/0.7; 0.5x tube lense. Airyscan SR mode

Kidney Tubule Organoids grown from mIMCD3 cells. Plan-APOCHROMAT 20x/0.7; 0.5x tube lens. Airyscan SR mode.

Celldiscoverer 7 enables confocal image acquisition of large scanning fields at high resolution. Imaging of tubular structures in multiwell plates is accomplished at unprecedented speed, allowing for high-throughput applications such as drug screening.

HeLa cells infected with Shigella flexneri expressing mCherry at 3 hours post infection to investigate cytoskeleton rearrangements during intracellular infection eg. formation of septin cages, actin tails. The sample was stained with DAPI (blue, nuclei), anti-SEPT7 antibodies (green, septin filaments), and phalloidin (magenta, F-actin). Maximum Intensity Projection acquired using the Celldiscoverer 7 using the 50x water object and the 0.5x magnification optics. Sample courtesy of A. López Jiménez, London School of Hygiene and Tropical Medicine, UK.

Zebrafish line Tg(lyz::dsRed). Observing emergency granulopoiesis in a 4 day post-fertilisation zebrafish embryo. The innate immune system induces this response to replenish the neutrophil (dsRed) population 48 hours post infection with a non-lethal dose of Shigella flexneri. Maximum Intensity Projection acquired using the Celldiscoverer 7 in widefield mode. Sample courtesy of M. Gomez, London School of Hygiene and Tropical Medicine, UK.

Sample courtesy of J. Hartmann and D. Gilmour, EMBL, Heidelberg, Germany

Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo (Danio rerio). Animals were anesthetized and embedded using low concentrated agarose in a glass bottom petridish.

Maximum intensity projections of the lateral line primordium tip migrating through the animal (155 z-planes).

Green: LYN-eGFP (mebranes); Red: tagRFP-T-UTRCH (actin).

 

Sample courtesy of S. Gawrzak and M. Jechlinger, EMBL, Heidelberg, Germany

Organoid from a human breast cancer cell line. The cells express GFP-labeled H2B (nucelei) and mCherry (cytoplasmic staining depicted here in grey for better visualization).

Several organoids where grown in a multiwell plate with Matrigel. Initial sample navigation was performed using the transmitted light at a low magnification of 2.5× to identify interesting organoids.

Subsequently, high resolution images were acquired using the water immersion objective with a total magnification of 50×. 61 z-planes were acquired using ZEISS Celldiscoverer 7 with LSM 900 and Airyscan 2 in Multiplex mode.

Sample courtesy of A.C. Hocke, Chartié, Berlin, Germany.

Human lung epithelial cell line A549 stained with MitoTracker™ Orange (mitochondiria) and SIR-DNA (nuclei).

The acquisition seamlessly combines two imaging modes – the fluorescent channels were captured in confocal mode using highly sensitive GaAsP detectors while the Phase Gradient Contrast is camera based.

Resources

ZEISS Celldiscoverer 7 – White Paper

The transformative impact at London School of Hygiene & Tropical Medicine

ZEISS Celldiscoverer 7

Your Automated Platform for Live Cell Imaging

pages: 50
file size: 14418 kB

ZEISS Celldiscoverer 7 - Application Note

High quality LSM imaging with autocorrection objectives using plastic labware

pages: 8
file size: 2151 kB

Automating Cellular Imaging with ZEISS Celldiscoverer 7

Technology Note

file size: 2550 kB

Learn more about the ZEISS Celldiscoverer 7

Contact Us

Explore The Possibilities Today by Filling Out the Form Below or Calling 01223 679779

 

Get in touch to discuss how the Celldicoverer 7 can change your research and register your interest.