ZEISS Lattice Lightsheet 7

Join us at the UCL-ZEISS Multiscale Imaging Centre to learn about and experience our latest technology

The ZEISS Lattice Lightsheet 7 couples cutting edge live cell imaging capabilities with the ease of use you expect from ZEISS. Redefining imaging speed to volumes per second and offering unrivalled gentleness for your live cell experiments. The volumetric imaging at subcellular resolution allows exquisite exploration of dynamics in live samples. The system has been specially designed to work with a range of standard sample carriers, such as slides, dishes and chamber slides, letting you focus on the science, instead of the sample preparation.

Seminar: How to tame a Lattice Lightsheet

Join us as we introduce the ZEISS Lattice Lightsheet 7 and its features. Learn about the lattice lightsheet principle and its advantages for 3D imaging of subcellular dynamics. We’ll provide insights into the technologies development process and delve into what the Lattice Lightsheet 7 can deliver for your applications.

Date: Tuesday 14th September 2021
Time: 13:00-14:00
Speaker: Chris Power, Product & Application Sales Specialist, ZEISS

Demo: Experience what the Lattice Lightsheet 7 can offer

Today’s research environment is highly dynamic and demands meaningful insights within ambitious time frames. The Lattice Lightsheet 7 offers unprecedented capabilities unique to the market, opening doors for a range of cutting edge science to be performed..

We’ll be offering a limited number of demonstrations on the system as follows:

Dates: Thursday 16th – Thursday 30th September 2021

Location:

UCL-ZEISS Multiscale Imaging Centre
21 University Street
Rockefeller Building
London
WC1E 6JJ

Closing date for demo requests: Thursday 9th September 2021

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Typical Applications

Live Cell Imaging

Volumetric imaging of

  • Subcellular processes: organelle morphology and dynamics, organelle-organelle interactions, vesicle trafficking
  • Membrane dynamics
  • Immune cells such as T cell mobility and activation
  • Gentle imaging of live cells for hours up to days with minimal phototoxicity and photobleaching
  • Cell proliferation and apoptosis assays

3D Cell Imaging

  • Live imaging of spheroids or organoids with diameters up to 200 μm
  • Organoid self-organization
  • Cell migration and proliferation within organoids
  • Imaging of cell-cell interactions, 3D organization, migration and morphology
  • In vitro imaging of neuronal activity

Small Evolving Organisms

  • Resolving structural detail in 3D with close to isotropic resolution
  • Fast imaging of cellular and subcellular dynamics in embryos and small organisms up to 100 μm in diameter
  • Cell migration, cell-cell interaction, cell cycle, vesicle trafficking

Oocytes

  • Live imaging of whole oocytes in 3D with subcellular detail

ZEISS Lattice Lightsheet 7 at Work

 

Human induced pluripotent stem cells which endogenously express mEGFP-tagged lamin B1 (AICS-0013). Images generated using AICS-0013 (LMNB1-mEGFP) from the Allen Institute for Cell Science. The overnight experiment was recorded for close to 8 hours with one volume imaged every 1.5 min.

Lamin B1 is involved in disassembling and reforming the nuclear envelope during mitosis. The formation of so-called ‘nuclear invaginations’ has been reported frequently during mitotic events. However, most research so far on these unique structures has been done with fixed cells and as a result their function is largely unknown.

The data observes cells going through mitosis throughout the whole duration. The formation and dynamics of nuclear invaginations can clearly be observed in most of the cells, throughout the complete cell cycle. The gentleness of Lattice Lightsheet 7 imaging enables imaging of mitotic events over longer time periods, preventing arrest from excitation light. Combined with the fast volumetric imaging and near-isotropic resolution the ZEISS Lattice Lightsheet 7 is the perfect tool for challenging experiments like this.

 

T cell expressing Lifeact-GFP. Color-coded depth projection and maximum intensity projection side-by-side. The T cell was imaged constantly for over 1 hr; one volume every 2.5 secs. Sample courtesy of M. Fritzsche, University of Oxford, UK

Register Your Place

Learn more about the Lattice Lightsheet 7

To register for a demo please complete the form, ticking the demo box, and tell us as much information about your samples as possible:

  • Sample type
  • Live or Fixed?
  • Fluoresence Staining
  • Sample Vessel