The ZEISS Lattice Lightsheet 7 couples cutting edge live cell imaging capabilities with the ease of use you expect from ZEISS. Redefining imaging speed to volumes per second and offering unrivalled gentleness for your live cell experiments. The volumetric imaging at subcellular resolution allows exquisite exploration of dynamics in live samples. The system has been specially designed to work with a range of standard sample carriers, such as slides, dishes and chamber slides, letting you focus on the science, instead of the sample preparation.
Seminar: Lattice Lightsheet 7
We’d like to introduce you to the ZEISS Lattice Lightsheet 7 and its features. In our bespoke seminar for your facility, learn about the Lattice Lightsheet principle and its advantages for 3D imaging of subcellular dynamics. We’ll provide insights into the technology’s development process and delve into what the ZEISS Lattice Lightsheet 7 can deliver for your applications.
Location: Your Campus
Speaker: Jonathan Shewring, Product & Sales Support Specialist, ZEISS
- Learn how lattice lightsheet technology is providing access to rich volumes of data with exciting scientific possibilities across samples as diverse as cells, embryos and plants
- See for yourself how easily you can generate volumetric datasets during the demo of the Lattice Lightsheet 7
- Take a look behind the scenes at the unique and impressive optical design that underlies the inverted implementation. Come prepared with your optics questions as we explore this unique capability!
The Lattice Lightsheet 7 enables you to address questions that have previously been unattainable using other light microscopy imaging techniques. Explore below to learn more about the typical applications the Lattice Lightsheet technology is suited to.
Live Cell Imaging
- Volumetric imaging of subcellular processes: organelle morphology and dynamics, organelle-organelle interactions, vesicle trafficking
- Volumetric Imaging of membrane dynamics
- Immune cells such as T cell mobility and activation
- Gentle imaging of live cells for hours up to days with minimal phototoxicity and photobleaching
- Cell proliferation and apoptosis assays
3D Cell Imaging
- Live imaging of spheroids or organoids with diameters up to 200 μm
- Organoid self-organization
- Cell migration and proliferation within organoids
- Imaging of cell-cell interactions, 3D organization, migration and morphology
- In vitro imaging of neuronal activity
Small Evolving Organisms
- Resolving structural detail in 3D with close to isotropic resolution
- Fast imaging of cellular and subcellular dynamics in embryos and small organisms up to 100 μm in diameter
- Cell migration, cell-cell interaction, cell cycle, vesicle trafficking
- Live imaging of whole oocytes in 3D with subcellular detail
Human induced pluripotent stem cells which endogenously express mEGFP-tagged lamin B1 (AICS-0013). Images generated using AICS-0013 (LMNB1-mEGFP) from the Allen Institute for Cell Science. The overnight experiment was recorded for close to 8 hours with one volume imaged every 1.5 min.
Lamin B1 is involved in disassembling and reforming the nuclear envelope during mitosis. The formation of so-called ‘nuclear invaginations’ has been reported frequently during mitotic events. However, most research so far on these unique structures has been done with fixed cells and as a result their function is largely unknown.
The data observes cells going through mitosis throughout the whole duration. The formation and dynamics of nuclear invaginations can clearly be observed in most of the cells, throughout the complete cell cycle. The gentleness of Lattice Lightsheet 7 imaging enables imaging of mitotic events over longer time periods, preventing arrest from excitation light. Combined with the fast volumetric imaging and near-isotropic resolution the ZEISS Lattice Lightsheet 7 is the perfect tool for challenging experiments like this.
T cell expressing Lifeact-GFP. Color-coded depth projection and maximum intensity projection side-by-side. The T cell was imaged constantly for over 1 hr; one volume every 2.5 secs. Sample courtesy of M. Fritzsche, University of Oxford, UK
Get in touch discuss how the Lattice Lightsheet 7 can change your research and register your interest for an onsite seminar.