ZEISS Lattice Lightsheet 7

Long-term volumetric imaging of living cells

The ZEISS Lattice Lightsheet 7 couples cutting edge live cell imaging capabilities with the ease of use you expect from ZEISS. Redefining imaging speed to volumes per second and offering unrivalled gentleness for your live cell experiments. The volumetric imaging at subcellular resolution allows exquisite exploration of dynamics in live samples. The system has been specially designed to work with a range of standard sample carriers, such as slides, dishes and chamber slides, letting you focus on the science, instead of the sample preparation.

Discover the Subcellular Dynamics of Life

Auto-aligning System

Focus your full attention on your experiments

Near-isotropic Resolution

Reveal three-dimensional details in their true proportions

High-speed Volumetric Imaging

Don’t miss an interesting event on your coverslip

Amazingly Simple Access

Examine living specimens directly on your standard sample carriers

Next to no Photo Damage

Watch the subcellular dynamics of life over hours and even days


Lattice Light Sheet Technology Made Accessible to Everyone

Watch our demonstration in under 60 seconds

The automatic alignments and easy workflows provided by the inverted Lattice Lightsheet 7 instrument mean that every user can now access this cutting-edge approach and capture 3D data of their classically mounted samples over hours and days at a time. Click the video to see the Lattice Lightsheet 7 demonstrated in less than 60 seconds.

 

Typical Applications

The Lattice Lightsheet 7 enables you to address questions that have previously been unattainable using other light microscopy imaging techniques. Explore below to learn more about the typical applications the Lattice Lightsheet technology is suited to.

Live Cell Imaging

  • Volumetric imaging of subcellular processes: organelle morphology and dynamics, organelle-organelle interactions, vesicle trafficking
  • Volumetric Imaging of membrane dynamics
  • Immune cells such as T cell mobility and activation
  • Gentle imaging of live cells for hours up to days with minimal phototoxicity and photobleaching
  • Cell proliferation and apoptosis assays

3D Cell Imaging

  • Live imaging of spheroids or organoids with diameters up to 200 μm
  • Organoid self-organization
  • Cell migration and proliferation within organoids
  • Imaging of cell-cell interactions, 3D organization, migration and morphology
  • In vitro imaging of neuronal activity

Small Evolving Organisms

  • Resolving structural detail in 3D with close to isotropic resolution
  • Fast imaging of cellular and subcellular dynamics in embryos and small organisms up to 100 μm in diameter
  • Cell migration, cell-cell interaction, cell cycle, vesicle trafficking

Oocytes

  • Live imaging of whole oocytes in 3D with subcellular detail

From Image to Results

Image analysis? No Problems

The Lattice Lightsheet 7 delivers ease of use for your cutting edge scientific questions, but what about the data? With arivis you can explore and analyse the data rich images from the Lattice Lightsheet 7 in a streamlined way. arivis Vision4D enables users to define and optimise complex image analysis pipelines using easy to navigate tools increasing the efficiency of your research. Data proficiency at everyone’s fingertips.

In our new series “From Image to Results” explore how data captured with the ZEISS Lattice Lightsheet 7 can be combined with a powerful Image Analysis Pipeline delivered by arivis Vision4D to generate a high-resolution longitudinal study of vesicle trafficking in Cos7 cells.

 

Trafficking mRNA molecules were tracked in arivis Vision4D®. The movement of the zebrafish embryo was first corrected using a nucleus reference track. Then individual mRNA molecules were tracked over time to result statistics such as speed and directionality. Sample: courtesy of Prof. Andrew Oates, EPFL, Switzerland.

ZEISS Lattice Lightsheet 7 at Work

 

Human induced pluripotent stem cells which endogenously express mEGFP-tagged lamin B1 (AICS-0013). Images generated using AICS-0013 (LMNB1-mEGFP) from the Allen Institute for Cell Science. The overnight experiment was recorded for close to 8 hours with one volume imaged every 1.5 min.

Lamin B1 is involved in disassembling and reforming the nuclear envelope during mitosis. The formation of so-called ‘nuclear invaginations’ has been reported frequently during mitotic events. However, most research so far on these unique structures has been done with fixed cells and as a result their function is largely unknown.

The data observes cells going through mitosis throughout the whole duration. The formation and dynamics of nuclear invaginations can clearly be observed in most of the cells, throughout the complete cell cycle. The gentleness of Lattice Lightsheet 7 imaging enables imaging of mitotic events over longer time periods, preventing arrest from excitation light. Combined with the fast volumetric imaging and near-isotropic resolution the ZEISS Lattice Lightsheet 7 is the perfect tool for challenging experiments like this.

 

T cell expressing Lifeact-GFP. Color-coded depth projection and maximum intensity projection side-by-side. The T cell was imaged constantly for over 1 hr; one volume every 2.5 secs. Sample courtesy of M. Fritzsche, University of Oxford, UK

 

Video: LLC-PK1 cell undergoing mitosis. Cells are expressing H2B-mCherry (cyan) and α-Tubulin mEGFP (magenta), recording over a period of 25 hours.

 

Cos 7 cells transiently transfected with CalnexinmEmerald and EB3-tdTomato. EB3 labels the growing ends of microtubules and is necessary for the regulation of microtubule dynamics. Calnexin is a protein of the ER where proteins are synthesized. Cells were imaged for 1.5 hrs every 80 secs, imaged volume: 175 × 120 × 70 μm³.

 

Spheroids and organoids are in vitro models of organs – much smaller and simpler but easy to produce and thus for developmental biologists an invaluable tool to study organ development. Unlike cell cultures, which usually consist of a monolayer of cells only, cells in spheroids / organoids form three-dimensional structures, allowing for the investigation of cell migration and differentiation inside 3D cell models. With lattice light sheet microscopy, imaging the development and self-organization of organoids becomes reality. Here, we can see a 3D rendering of a spheroid consisting of cells expressing H2B-mCherry (cyan) and α-Tubulin-mEGFP (magenta). Not every cell is labelled.

 

DeltaD-YFP transgenic zebrafish embryo (Liao et al. 2016, Nature Communications). Fusion protein driven by a transgene containing the endogenous regulatory regions, expression in the tailbud and pre-somitic mesoderm. Signal visible in the cell cortex, and in puncta corresponding to trafficking vesicles (green). Nuclei in magenta. The embryo was imaged for 5 minutes constantly; one volume (150 × 50 × 90 μm3) every 8 sec. Sample: courtesy of Prof. Andrew Oates, EPFL, Switzerland.

Resources

ZEISS Lattice Lightsheet 7

Long-term Volumetric Imaging of Living Cells

Pages: 28
File size: 3624 kB

Learn more about the ZEISS Lattice Lightsheet 7

Contact Us

Explore The Possibilities Today by Filling Out the Form Below or Calling 01223 679779

Learn more about the Lattice Lightsheet 7

Get in touch discuss how the Lattice Lightsheet 7 can change your research and register your interest for an onsite seminar or demo.

Evolution of Lattice Lightsheet Technology:

Key Milestones

December 2011

First exposure using the ground-breaking homebuilt Lattice Lightsheet system, developed by Eric Betzig and his team at Janelia Research Campus.

November 2013

A concept is developed for an inverted system configuration and compatibility with standard samples.

September 2014

ZEISS and the Howard Hughes Medical Institute’s Janelia Research Campus sign an exclusive license agreement for the commercialisation of Lattice light sheet microscopy and development of a turnkey ZEISS system.

October 2014

Eric Betzig is jointly awarded The Nobel Prize in Chemistry for “the development of super-resolved fluorescence microscopy” and landmark paper “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution” is published in Science Magazine.

September 2016

First biological data is acquired from demonstrator setup, at Carl Zeiss Microscopy, Jena.

November 2017

Product engineering begins.
1. Implementation of a range of optical improvements through the development of autoimmersion, transmission and aberration correction features.
2. Development of software for improved system control, processing, data handling and auto alignment.

November 2019

Beta testing programme begins at nine sites worldwide, with pre-serial systems placed in the United States, Australia, Germany, Switzerland, Sweden and the Netherlands.

December 2020

Official launch of the series system ZEISS Lattice Lightsheet 7, making Lattice Light Sheet technology accessible to everyone - with its automatic alignments, easy workflows, inverted design and compatibility with standard sample carriers.

July 2021

University of Oxford trailblaze with the first official installation of the ZEISS Lattice Lightsheet 7 globally, in a project led by Marco Fritzsche, Associate Professor and Rosalind Franklin Kennedy Trust Research Fellow at The Kennedy Institute of Rheumatology.