ZEISS Lattice Lightsheet 7

Long-term volumetric imaging of living cells

The ZEISS Lattice Lightsheet 7 couples cutting edge live cell imaging capabilities with the ease of use you expect from ZEISS. Redefining imaging speed to volumes per second and offering unrivalled gentleness for your live cell experiments. The volumetric imaging at subcellular resolution allows exquisite exploration of dynamics in live samples. The system has been specially designed to work with a range of standard sample carriers, such as slides, dishes and chamber slides, letting you focus on the science, instead of the sample preparation.

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Lattice Lightsheet 7: Advanced and Novel Capabilities for 21ALERT

The Lattice Lightsheet 7 offers unprecedented capabilities unique to the market. The ability to interpret cellular processes within their native microenvironment is a critical factor to advance the understanding of processes which govern life and will determine outcomes across numerous fields of society. This opens doors for a range of cutting edge science to be performed and offers the strong basis for a BBSRC 2021 Alert applications.

Discover the Subcellular Dynamics of Life

Auto-aligning System

Focus your full attention on your experiments

Near-isotropic Resolution

Reveal three-dimensional details in their true proportions

High-speed Volumetric Imaging

Don’t miss an interesting event on your coverslip

Amazingly Simple Access

Examine living specimens directly on your standard sample carriers

Next to no Photo Damage

Watch the subcellular dynamics of life over hours and even days


Typical Applications

Live Cell Imaging

  • Volumetric imaging of subcellular processes: organelle morphology and dynamics, organelle-organelle interactions, vesicle trafficking
  • Volumetric Imaging of membrane dynamics
  • Immune cells such as T cell mobility and activation
  • Gentle imaging of live cells for hours up to days with minimal phototoxicity and photobleaching
  • Cell proliferation and apoptosis assays

3D Cell Imaging

  • Live imaging of spheroids or organoids with diameters up to 200 μm
  • Organoid self-organization
  • Cell migration and proliferation within organoids
  • Imaging of cell-cell interactions, 3D organization, migration and morphology
  • In vitro imaging of neuronal activity

Small Evolving Organisms

  • Resolving structural detail in 3D with close to isotropic resolution
  • Fast imaging of cellular and subcellular dynamics in embryos and small organisms up to 100 μm in diameter
  • Cell migration, cell-cell interaction, cell cycle, vesicle trafficking

Oocytes

  • Live imaging of whole oocytes in 3D with subcellular detail

ZEISS Lattice Lightsheet 7 at Work

 

Human induced pluripotent stem cells which endogenously express mEGFP-tagged lamin B1 (AICS-0013). Images generated using AICS-0013 (LMNB1-mEGFP) from the Allen Institute for Cell Science. The overnight experiment was recorded for close to 8 hours with one volume imaged every 1.5 min.

Lamin B1 is involved in disassembling and reforming the nuclear envelope during mitosis. The formation of so-called ‘nuclear invaginations’ has been reported frequently during mitotic events. However, most research so far on these unique structures has been done with fixed cells and as a result their function is largely unknown.

The data observes cells going through mitosis throughout the whole duration. The formation and dynamics of nuclear invaginations can clearly be observed in most of the cells, throughout the complete cell cycle. The gentleness of Lattice Lightsheet 7 imaging enables imaging of mitotic events over longer time periods, preventing arrest from excitation light. Combined with the fast volumetric imaging and near-isotropic resolution the ZEISS Lattice Lightsheet 7 is the perfect tool for challenging experiments like this.

 

T cell expressing Lifeact-GFP. Color-coded depth projection and maximum intensity projection side-by-side. The T cell was imaged constantly for over 1 hr; one volume every 2.5 secs. Sample courtesy of M. Fritzsche, University of Oxford, UK

Resources

ZEISS Lattice Lightsheet 7

Long-term Volumetric Imaging of Living Cells

Pages: 28
File size: 3624 kB

Learn more about the ZEISS Lattice Lightsheet 7

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Strengthen your BBSRC Application with ZEISS

 

To help you streamline and strengthen your BBSRC application with a strong Industry partner collaboration, consult with ZEISS personnel on the requirements pertaining to your proposal or microscopy instruments and solutions that may be well-suited to the scope of the program.