ZEISS Celldiscoverer 7 with LSM 900 and Airyscan 2

Your automated microscope for gentle and fast confocal 4D imaging

With Celldiscoverer 7, you combine the ease-of-use of an automated high-end imaging system with the image quality and flexibility of a classic inverted research microscope. Celldiscoverer 7 calibrates itself, detects and focuses on your samples while the optics adjust themselves.

Life sciences research often calls for optical sectioning to image samples with best possible contrast and resolution. The Celldiscoverer 7 with LSM 900 and Airyscan 2 enables gentle 3D imaging of dynamic processes in your living samples with highest framerates in superresolution and easy separation of multiple labels with spectral imaging.

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Celldiscoverer 7 with LSM 900 and Airyscan 2: Advanced and Novel Capabilities for 21ALERT

Multimodal imaging is a key trend for microscopy in Life Sciences research, offering the ability to holistically interrogate cells, tissues and organisms across multiple scales is key for both basic and translational research. With the Celldiscoverer 7 it’s never been easier to precisely connect widefield, confocal and superresolution images. Fast multimodal acquisition simplifies and speeds up your workflow which providing unique contextual insights into samples. Automation of this workflow in todays world enables vital research to continue remotely from site via remote access. The capabilities of the Celldiscoverer 7 form a strong basis for the BBSRC 2021 Alert Call.

Reproducible Results Made Easy

Find Focus – Keep Focus

A hardware-based focus finds and keeps the focus automatically after detecting the thickness and optical properties of the sample carrier for demanding long-term, time-lapse imaging.

Multimodal imaging

A system with a fast, sensitive sCMOS camera combined with the LSM 900 and Airyscan 2. Ensuring a suitable modality to suit an array of biological questions.

Easily Get Reproducible Results

Automatic calibration routines ensure reproducible results in live cell imaging.

Autoimmersion

Celldiscoverer 7 combines an outstanding 50x water immersion objective with rapid automated immersion supply and removal. Automatic monitoring of immersion and adding water, as needed

Automatic Sample Recognition

You’re free to use Petri dishes, chamber slides, multiwell plates, plastic or glass, thin or thick vessel bottoms, low skirt or high skirt plates. Automatic sample recognition detects vessel features and calibrates the microscope for optimal performance.

Autocorr Objectives

Autocorr objectives automatically adjust the correct collar to correct spherical aberrations delivering you crisp contrast and high resolution

Typical Applications

Live Cell Imaging

  • Short-term or long-term observation of physiological and morphological parameters in 2D/ 3D during growth, differentiation, motility and interaction.
  • Acquire high resolution images of multi-labelled cell culture from multiwell plates quickly.
  • Study the motility of vesicles, organelles and subcellular structures
  • Examine the interplay of multiple proteins
  • Image subcellular structures at physiological expression levels

  • Study molecular dynamic with FRAP
  • Explore the interaction of two proteins with FRET
  • Observation of stimulus-induced responses of cells or organisms without disturbing the environmental control.
  • Perform label free growth curve assays over several days

3D Cell Imaging

  • Live imaging of spheroids or organoids
  • Organoid self-organization
  • Cell migration and proliferation within organoids
  • Study morphogenetic process using tubule organoids.

Small Evolving Organisms

  • Cell migration and proliferation within organoids
  • Study morphogenetic process using tubule organoids.
  • Analyse the embryogenesis of small model organisms

Fixed Specimens

  • Change quickly between large overview scans and high resolution imaging
  • Fixed fluorescently labelled tissue, cell culture samples - Identification, quantification and qualification of cell types, pathological and pharmacological pathways using cell-, tissue and protein markers in 2D and 3D samples.
  • Label-free fixed and thin tissue slices or small organisms

ZEISS Celldiscoverer 7 at Work

Sample courtesy of J. Hartmann and D. Gilmour, EMBL, Heidelberg, Germany

Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo (Danio rerio). Animals were anesthetized and embedded using low concentrated agarose in a glass bottom petridish.

Initial camera based imaging allowed for a quick and easy sample navigation (top) combining Phase Gradient Contrast with fluorescence acquisition.

Subsequent high resolution imaging with Airyscan 2 in Multiplex mode was done on individual positions identified in the widefield image (white boxes).
A) Maximum intensity projections of an immature neuromast (127 z planes).
B) Maximum intensity projections of the lateral line primordium tip migrating through the animal (155 z-planes).

Green: LYN-eGFP (mebranes);
Red: tagRFP-T-UTRCH (actin).

The gentle and fast image acquisition that is inherent to the Airyscan 2 Multiplex mode is very benificial for this kind of application. The animal is unperturbed by the imaging while images with a very high signal to noise ratio as well as level of detail can be acquired at the same time.

© Courtesy of S. Gawrzak and M. Jechlinger, EMBL, Heidelberg, Germany

Organoid from a human breast cancer cell line. The cells express GFP-labeled H2B (nucelei) and mCherry (cytoplasmic staining depicted here in grey for better visualization).

Several organoids where grown in a multiwell plate with Matrigel. Initial sample navigation was performed using the transmitted light at a low magnification of 2.5× to identify interesting organoids.

Subsequently, high resolution images were acquired using the water immersion objective with a total magnification of 50×. 61 z-planes were acquired using ZEISS Celldiscoverer 7 with LSM 900 and Airyscan 2 in Multiplex mode.

One can clearly appreciate the robustness of the imaging given that Matrigel is not an ideal optical medium and the organoid was imaged at a distance of several micrometers from the coverglass
 

Resources

ZEISS Celldiscoverer 7

Your Automated Platform for Live Cell Imaging

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Strengthen your BBSRC Application with ZEISS

 

To help you streamline and strengthen your BBSRC application with a strong Industry partner collaboration, consult with ZEISS personnel on the requirements pertaining to your proposal or microscopy instruments and solutions that may be well-suited to the scope of the program.