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3D view of an segmented intestinal organoid imaged with ZEISS Volutome. Cutting and imaging parameters of the original stack: Dimensions: 65 x 65 x 12 µm , Pixel size: 6 nm, Cutting thickness 40 nm (300 sections). EHT: 1,5 kV / Ip: 100pA, Dwell time: 1 µsSample Courtesy of Alexandra Graff-Meyer and Marc Buehler, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
Sample Courtesy of Alexandra Graff-Meyer and Marc Buehler, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

ZEISS Volume Electron Microscopy

User Group Meeting & Workshop
University of Liverpool, Biomedical EM Facility
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3D view of an segmented intestinal organoid imaged with ZEISS Volutome. Sample Courtesy of Alexandra Graff-Meyer and Marc Buehler, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
Sample Courtesy of Alexandra Graff-Meyer and Marc Buehler, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland

3D view of an segmented intestinal organoid imaged with ZEISS Volutome. Segmentation of nuclei (green), mitochondria (cyan), microvilli (orange), nucleus (blue) and granules (brown) within the 3D dataset.

Cutting and imaging parameters of the original stack:
Dimensions: 65 x 65 x 12 µm , Pixel size: 6 nm, Cutting thickness 40 nm (300 sections). EHT: 1,5 kV / Ip: 100pA, Dwell time: 1 µs

Overview

Join us for two days of hands-on learning, expert talks, and community building around Volume Electron Microscopy (vEM). Whether you’re already using Volutome or exploring vEM workflows, this event is designed to energize your projects and deepen your expertise.

What to Expect

  • Live Demonstrations: Volutome in action, alignment workflows, and sectioning setups
  • User Talks: Hear from leading researchers on their journey with AT and SBF workflows
  • Networking: Connect with peers and ZEISS experts

Topics

  • Arivis cloud segmentation and data handling
  • AT workflow journeys
  • SBF workflows and Hands-on with Volutome
  • Sectioning for AT and Alignment workflows
  • STEM Array insights

Agenda

Date and Time

Session Title

Day 1 (11:00 - 16:30)

Welcome and AT Sessions

Lunch

Afternoon Sessions

Evening Meal

Day 2 (09:00 - 12:00)

Volutome Sessions

Closing Discussions
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Venue and Logistics

University of Liverpool, Biomedical EM Facility, Nuffield Wing

Biomedical EM Facility, Nuffield Wing University of Liverpool L69 3BX Liverpool United Kingdom
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Accommodation:
Holiday Inn near Lime Street. (group rate pending)


Catering:
Lunch will be provided and there will be an evening meal on the first day.

Reserve Your Spot

Complete this short form to register and reserve your spot - spaces are limited!
3D image of a segmented mouse brain Volutome dataset Sample Courtesy of Christel Genoud, Université de Lausanne, EMF, 1015 Lausanne, Switzerland
Sample Courtesy of Christel Genoud, Université de Lausanne, EMF, 1015 Lausanne, Switzerland

3D image of a segmented mouse brain Volutome dataset. 10 segmented main neurons out of the volume and a blood vessel (red).
Cutting and Imaging Parameters: Pixel size: 6 nm, Cutting thickness: 25 nm, Dimensions: 43 μm × 43 μm × 45 μm (1800 sections), EHT: 1.2 kV / Ip: 90 pA, Dwell time: 0.8 and 1.6 μs, respectively, acquired with ZEISS GeminiSEM 460
AI based segmentation of the displayed neurons and final proofreading: scalableminds.com
Visualization: arivis pro

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