Event

Unlock the Power of Cryo Microscopy:

A Technical Workshop in Partnership With UCD

Wed, 11 December 2024

Join leading researchers to explore cutting-edge cryo CLEM, cryo FIB tomography, and cryo TEM lamella preparation.

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Overview

This workshop provides a unique opportunity to learn advanced cryo microscopy techniques, including cryo CLEM, cryo FIB tomography, and cryo TEM lamella preparation, used to analyse samples in their near-native state. These workflows are key in generating high-resolution, 3D imaging of biological samples, offering insights at the nanoscale.

Learn more about these techniques, get support from experienced ZEISS specialists and exchange knowledge with your fellow researchers. Limited spots available — register early!

Date: Wednesday 11^th December 2024
Venue: Charles Seminar Room and KB Gallery, Charles Institute Building, University College Dublin

Complimentary lunch included for all attendees

Why You Shouldn't Miss This Workshop

Learn the fundamentals of cryo microscopy research using light, electron and X-ray techniques
Gain insights into available imaging platforms, workflows and samples
Discover how cryo microscopy techniques apply to a broad range of biological research questions
Ask your questions to leading cryo experts
We will explore the challenges of cryo correlative microscopy and how to overcome them

Agenda

Time	Session Title	Session Description
9:00 - 9:45	Arrival & Networking
9:45 - 10:00	Introduction	Meeting objectives and ZEISS introduction
10:00 - 11:00	Cryo Research and 3D Tomography	Cryo Research Community 3D Tomography Ambient Cryo Tomography Cryo lamella preparation and lift out Correlation CLEM
11:00 - 11:30	Q&A and Coffee Break
11:30 - 12:00	Sirius XT Cryo CT Presentation
12:00 - 12:45	Lunch
12:45 - 13:30	UCD Cryo Research Focus Topics	Impact of cryo on lab reasearch at UCD
13:30 - 14:30	Innovative Cryo Exchange	Open discussion around sample transfer, CT transfer and correlation, Cryo CLXM workflows, Volumetric, Lamella, CLEM and correlation with X-ray. Q&A

Speaker Dr. Raffaella Carzaniga Application and Product Specialist for Bio Electron Microscopy (Life Sciences), Carl Zeiss Ltd

Raffaella is a Business Development Application and Product Specialist for Bio Electron Microscopy (Life Sciences). She joined ZEISS after a successful career in academia where she built and managed several prestigious scientific imaging platforms.

Her experience spans a wide range of multi modalities and workflows. Her great passion for knowledge transfer and training translates into creating customer-tailored solutions with the ZEISS imaging portfolio.

Speaker Dr. Andreas Schertel Staff Scientist and Global Application Support Crossbeam, Carl Zeiss Microscopy GmbH

Andreas Schertel works as staff scientist for FIB-SEM microscopy at the ZEISS Microscopy Customer Center Europe in Oberkochen. Andreas graduated in Physics and received his PhD in Physical Chemistry at the University of Heidelberg.

After working for a former manufacturer of Focused Ion Beam microscopes, he joined ZEISS in 2004. Andreas has more than 20 years of experience in the field of FIB-SEM microscopy and related applications. In recent years he has been focused on life science applications especially using cryogenic techniques.

Speaker Dr. Matt Haley Account Manager - North & ROI, Carl Zeiss Ltd

Dr. Matthew Haley has been with ZEISS for over 7 years. He is the Account Manager for the North West of England and Dublin.

Matthew has a PhD in Physiology and years of experience taking industry requirements and translating them into powerful research solutions. Matt is able to advise on the best microscopy solution to suit your research needs.

Speaker Dr. Raffaella Carzaniga Application and Product Specialist for Bio Electron Microscopy (Life Sciences), Carl Zeiss Ltd

Speaker Dr. Andreas Schertel Staff Scientist and Global Application Support Crossbeam, Carl Zeiss Microscopy GmbH

Speaker Dr. Matt Haley Account Manager - North & ROI, Carl Zeiss Ltd

Dr. Matthew Haley has been with ZEISS for over 7 years. He is the Account Manager for the North West of England and Dublin.

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We value your participation!
During the Q&A sessions, you will have the unique opportunity to engage directly with the ZEISS cryo team. To ensure a fruitful discussion, please share any questions or topics you would like to explore in advance. Your input will help us tailor the session to better meet your interests and needs.

Venue

University College Dublin

University College Dublin Charles Seminar Room, Charles Institute Building Dublin 4 Dublin Ireland

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In Partnership With University College Dublin

This workshop is held in partenership with the UCD Conway Imaging Core. The focus of the UCD Conway Imaging Core is to provide contemporary imaging technologies and know-how to researchers in the wider scientific community; both in academia and industry. The consolidated instrumentation suite spans all aspects of light and electron microscopy as well as sample preparation and image processing and analysis facilities. The Core team use their technical expertise to assist in the widest possible range of applications for microscopy, in advancing the development of new applications and indeed new imaging technologies.

Additional Information

Applications - ZEISS Correlative Cryo Workflow at Work

3D reconstruction of cellular structures
Overlay of a high-resolution LSM/Airyscan image

Courtesy of Kedar Narayan, National Cancer Institute / NIH and Frederick National Laboratory for Cancer Research, USA

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Courtesy of Kedar Narayan, National Cancer Institute / NIH and Frederick National Laboratory for Cancer Research, USA

3D reconstruction of cellular structures
Sample courtesy of M. Pilhofer, ETH Zürich, Switzerland

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Sample courtesy of M. Pilhofer, ETH Zürich, Switzerland

Overlay of a high-resolutionLSM/Airyscan image with a high-contrast Crossbeam image acquired under cryogenic conditions. This overlay was done with ZEN Connect.