ZEISS Lattice Lightsheet 7

Long-term volumetric imaging of living cells

The ZEISS Lattice Lightsheet 7 couples cutting edge live cell imaging capabilities with the ease of use you expect from ZEISS. Redefining imaging speed to volumes per second and offering unrivalled gentleness for your live cell experiments. The volumetric imaging at subcellular resolution allows exquisite exploration of dynamics in live samples. The system has been specially designed to work with a range of standard sample carriers, such as slides, dishes and chamber slides, letting you focus on the science, instead of the sample preparation.

Discover the Subcellular Dynamics of Life

Amazingly Simple Access

Examine living specimens directly on your standard sample carriers

Next to no Photo Damage

Watch the subcellular dynamics of life over hours and even days

Near-isotropic Resolution

Reveal three-dimensional details in their true proportions

Auto-aligning System

Focus your full attention on your experiments

High-speed Volumetric Imaging

Don’t miss an interesting event on your coverslip

Lattice Light Sheet Technology Made Accessible to Everyone

Watch our demonstration in under 60 seconds

The automatic alignments and easy workflows provided by the inverted Lattice Lightsheet 7 instrument mean that every user can now access this cutting-edge approach and capture 3D data of their classically mounted samples over hours and days at a time. Click the video to see the Lattice Lightsheet 7 demonstrated in less than 60 seconds.


Typical Applications

    • Short-term or long-term observation of physiological and morphological parameters in 2D/ 3D during growth, differentiation, motility and interaction.
    • Acquire high resolution images of multi-labelled cell culture from multiwell plates quickly.
    • Study the motility of vesicles, organelles and subcellular structures
    • Examine the interplay of multiple proteins
    • Image subcellular structures at physiological expression levels
    • Study molecular dynamic with FRAP
    • Explore the interaction of two proteins with FRET
    • Observation of stimulus-induced responses of cells or organisms without disturbing the environmental control.
    • Perform label free growth curve assays over several days
    • Live imaging of spheroids or organoids with diameters up to 200 μm
    • Organoid self-organization
    • Cell migration and proliferation within organoids
    • Imaging of cell-cell interactions, 3D organization, migration and morphology
    • In vitro imaging of neuronal activity
    • Resolving structural detail in 3D with close to isotropic resolution
    • Fast imaging of cellular and subcellular dynamics in embryos and small organisms up to 100 μm in diameter
    • Cell migration, cell-cell interaction, cell cycle, vesicle trafficking
    • Live imaging of whole oocytes in 3D with subcellular detail

From Image to Results

Image analysis? No Problems

The Lattice Lightsheet 7 delivers ease of use for your cutting edge scientific questions, but what about the data? With arivis you can explore and analyse the data rich images from the Lattice Lightsheet 7 in a streamlined way. arivis Vision4D enables users to define and optimise complex image analysis pipelines using easy to navigate tools increasing the efficiency of your research. Data proficiency at everyone’s fingertips.

Trafficking mRNA molecules were tracked in arivis Vision4D®. The movement of the zebrafish embryo was first corrected using a nucleus reference track. Then individual mRNA molecules were tracked over time to result statistics such as speed and directionality. Sample: courtesy of Prof. Andrew Oates, EPFL, Switzerland.

In our new series “From Image to Results” explore how data captured with the ZEISS Lattice Lightsheet 7 can be combined with a powerful Image Analysis Pipeline delivered by arivis Vision4D to generate a high-resolution longitudinal study of vesicle trafficking in Cos7 cells.

ZEISS Lattice Lightsheet 7 at Work

  • Human induced pluripotent stem cells which endogenously express mEGFP-tagged lamin B1 (AICS-0013). Images generated using AICS-0013 (LMNB1-mEGFP) from the Allen Institute for Cell Science. The overnight experiment was recorded for close to 8 hours with one volume imaged every 1.5 min.

    Lamin B1 is involved in disassembling and reforming the nuclear envelope during mitosis. The formation of so-called ‘nuclear invaginations’ has been reported frequently during mitotic events. However, most research so far on these unique structures has been done with fixed cells and as a result their function is largely unknown.

    The data observes cells going through mitosis throughout the whole duration. The formation and dynamics of nuclear invaginations can clearly be observed in most of the cells, throughout the complete cell cycle. The gentleness of Lattice Lightsheet 7 imaging enables imaging of mitotic events over longer time periods, preventing arrest from excitation light. Combined with the fast volumetric imaging and near-isotropic resolution the ZEISS Lattice Lightsheet 7 is the perfect tool for challenging experiments like this.

  • T cell expressing Lifeact-GFP. Color-coded depth projection and maximum intensity projection side-by-side. The T cell was imaged constantly for over 1 hr; one volume every 2.5 secs. Sample courtesy of M. Fritzsche, University of Oxford, UK

  • T cell expressing Lifeact-GFP. Color-coded depth projection and maximum intensity projection side-by-side. The T cell was imaged constantly for over 1 hr; one volume every 2.5 secs. Sample courtesy of M. Fritzsche, University of Oxford, UK

  • Cos 7 cells transiently transfected with CalnexinmEmerald and EB3-tdTomato. EB3 labels the growing ends of microtubules and is necessary for the regulation of microtubule dynamics. Calnexin is a protein of the ER where proteins are synthesized. Cells were imaged for 1.5 hrs every 80 secs, imaged volume: 175 × 120 × 70 μm³.

  • Spheroids and organoids are in vitro models of organs – much smaller and simpler but easy to produce and thus for developmental biologists an invaluable tool to study organ development. Unlike cell cultures, which usually consist of a monolayer of cells only, cells in spheroids / organoids form three-dimensional structures, allowing for the investigation of cell migration and differentiation inside 3D cell models. With lattice light sheet microscopy, imaging the development and self-organization of organoids becomes reality. Here, we can see a 3D rendering of a spheroid consisting of cells expressing H2B-mCherry (cyan) and α-Tubulin-mEGFP (magenta). Not every cell is labelled.

  • DeltaD-YFP transgenic zebrafish embryo (Liao et al. 2016, Nature Communications). Fusion protein driven by a transgene containing the endogenous regulatory regions, expression in the tailbud and pre-somitic mesoderm. Signal visible in the cell cortex, and in puncta corresponding to trafficking vesicles (green). Nuclei in magenta. The embryo was imaged for 5 minutes constantly; one volume (150 × 50 × 90 μm3) every 8 sec.  Sample: courtesy of Prof. Andrew Oates, EPFL, Switzerland.


    • ZEISS Lattice Lightsheet 7

      Long-term Volumetric Imaging of Living Cells

      Pages: 28
      File size: 3 MB
    • Lattice Lightsheet Technology

      Systems comparison table

      Pages: 1
      File size: 1 MB

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