ZEISS Elyra 7 with Lattice SIM²
Your Live Imaging System with Unprecedented Resolution
The super-resolution microscope Elyra 7 takes you far beyond the diffraction limit of conventional microscopy: With Lattice SIM² you can now double the conventional SIM resolution and discriminate the finest sub-organelle structures, even those no more than 60 nm apart. You don‘t need to sacrifice resolution when imaging at high speed using only the minimal exposure needed for life observation. Elyra 7 enables you to combine super-resolution and high-dynamic imaging – without the need for special sample preparation or expert knowledge of complex microscopy techniques.
SIM²: Double your SIM Resolution
What if you could push the resolution even further – down to 60 nm?
Lattice SIM opened the door to new applications by enabling gentle super-resolution imaging down to 120 nm at incredible high speed and with the ability to image deeper into challenging samples.
With Lattice SIM² you can now double the conventional SIM resolution.
SIM² is a novel image reconstruction algorithm that increases the resolution and sectioning quality of structured illumination microscopy data and raises the SIM technology to a new level. With SIM² you can now discriminate sub-organelle structures down to 60 nm without the need for special sample preparation or expert knowledge of complex techniques.
Watch the video to see the comparison of SIM to SIM² sectioning.
- Reveal mechanistic details in live cells, e.g. moving organelles, vesicle trafficking, membrane reorganisation
- Resolve structural details in 3D and multiple colours. Acquire up to four colours with optimized resolution for each wavelength.
- Discover fast cellular processes in the context of whole cells.
- Track many molecules over a large FOV and retrieve diffusion behaviour information in entire cells.
- Study molecular level structural changes of sub-minute-scale dynamic processes, e.g. mechanisms of focal adhesions, reorganization of tubulin, vesicle shuttling.
- Resolve structural detail in 3D with high penetration depth
- Resolve structural details in 3D over large areas using tiling
- Resolve structural detail in 3D with high penetration depth
- Probe the structural organization of a whole cell with the advantage of fluorescence specificity and superresolution.
- Investigate arrangement of cellular components and proteins
- Explore interaction of molecules.
- Reveal the ultrastructue of organelles and molecular assemblies
- Explore interaction of molecules
From Image to Results
Image analysis? No Problems
Translate your images into quantitative data
ZEN software provides you with a variety of different image pre- and post-processing tools. For example, you can take advantage of machine learning based on the ZEN Intellesis module to segment complex image data in an easy and intuitive way.
Use the efficient arivis Vision4D® software forvisualization and quantification of large 3D and 4D data sets imaged with your Elyra 7.
Vision4D® provides advanced image processing tools such as volume fusion, channel shift, conventional and machine learning based segmentation and 3D tracking. Visualize your quantitative results within the arivis Vision4D® software or export all data for further analysis.
ZEISS Elyra 7 at Work
SIM² Apotome: Comparison of widefield and SIM² Apotome single plane images of Cos-7 cells stained for microtubules (anti-alpha-tubulin Alexa Fluor 488, green) and nuclei (Hoechst, blue). Objective: LD LCI Plan-Apochromat 25× / 0.8 Imm Corr
SIM² Apotome: Flexible Optical Sectioning
The SIM Apotome acquisition mode in combination with the SIM² reconstruction algorithm now allows you to further tune the gentleness of your fast live-cell imaging with high contrast and resolution. Lattice SIM acquisition can be operated at 9 phase images per frame while 3 phase images per frame are sufficient for SIM Apotome, increasing the imaging speed by 44 % and 66 %, respectively.
Use your new optical sectioning speed to increase your productivity when acquiring large sample areas or large volumes at different magnifications
U2OS cell expressing Rab5-mEmerald (green) and tdTomato tagged Golgi associated transport marker (magenta). Simultaneous dual-color acquisition with an exposure time of 1.5ms / phase for a FOV of 1024 × 1024 pixel (64 µm × 64 µm).
Burst Mode - Super-resolution Imaging at up to 255 fps
The diffusive and especially the ballistic movement of small vesicles in cells can be captured only when super-resolution and high-dynamic imaging are possible at the same time. With the Burst processing of 2D time lapse data, Elyra 7 is able to generate super-resolution images at 255 Hz in a large field of view and even acquire two colors simultaneously in both Lattice SIM and SIM Apotome acquisition modes.
Three Times Faster Digital Sectioning
U2OS cell expressing calreticulin-tdTomato to visualize the endoplasmic reticulum. The time series shows a maximum intensity projection of the volume data set.
Elyra 7 Leap mode accelerates the volume imaging speed three times and at the same time decreases the light dosage on your sample. While still capturing all the finest details, the entire volume (18 planes) of the U2OS cell expressing Calreticulin-tdTomato was imaged at 38 volumes / min speed in Lattice SIM acquisition mode. For SIM Apotome acquisition mode, you can expect up to three times higher volume imaging speed.
Architecture of threefold labeled synaptonemal complexes from mouse testis visualized via immunolabeling of SYCP3 with SeTau647, SYCP1-C with Alexa 488 and SYCP1-N with Alexa 568 and Lattice SIM² mode
Multi-color Super-resolution Imaging for Conventionally Stained Samples
Due to its small size, three-color imaging of the synaptonemal complex has previously been possible only using complex methods like super-resolution imaging of three-fold expanded samples. Lattice SIM² resolves the two strands of SYCP3 (lateral elements) as well as SYCP1-C (C-terminus of transverse filaments) without special sample treatment or staining for distances well below 100 nm. More importantly, the three-color image provides structural information about the distances between the proteins SYCP3 and SYCP1. Even within the SYCP1 protein, the different labeled N- and C-Terminus can be clearly separated with less than 50 nm resolution between the two labels.
Single Molecule Localization Microscopy (SMLM)
SMLM encompasses techniques such as PALM, dSTORM, and PAINT. With high power lasers across the visible spectrum and dual camera detection, Elyra 7 allows researchers to gain access to a broad range of dyes, markers and fluorophores in almost any possible combination.
Resolve Molecular Structures
SMLM allows you to map precise locations of individual proteins.